Brett Tortelli

  • Slinger, WI

  • Wisconsin-Madison, U. of (2011)

  • Molecular Microbiology and Microbial Pathogenesis

  • Justin C. Fay, Ph.D.

  • Candida species and bacterial strains in the vaginal microbiome



The vaginal microbiome has important clinical implications as its composition has been associated with reproductive health and disease. Previous work had shown that the vaginal microbiome can be described by community types that are characterized by the abundance or absence of 4 commonface=inherit size=2 color="#201F1E"> size=2 color="#201F1E">Lactobacilluscolor="#201F1E"> color="#201F1E">species. face="Times New Roman" size=2>While recent studies have identified associations between community type and disease, these findings have not been consistent across study populations, possibly reflecting confounding aspects of the microbial community. My thesis work incorporatedface=inherit size=2> Candidaface=inherit size=2> and bacterial strain to provide a framework for a more holistic evaluation of the microbiome.face=inherit size=2> Using sequencing and molecular techniques, face="Times New Roman" size=2>I characterized the relationship betweenface=inherit size=2> Candida species and bacteria in the vagina. I identified differences amongface=inherit size=2> Lactobacillusface=inherit size=2> species in their association with vaginalface=inherit size=2> Candidaface=inherit size=2> colonization that correlate with species differences in lactic acid production. I also, developed a SNP-based computational approach using metagenomic sequencing data to characterize strain variation in vaginal bacteria. This work provided insight into the diversity, structure and evolutionary history of these bacterial species and led us to hypothesize that divergent strain groups could reflect different ecological species.

Last Updated: 8/25/2017 12:15:36 AM

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