Nicolas Ledru
Program: Molecular Genetics and Genomics
Current advisor: Benjamin D. Humphreys, MD, PhD
Undergraduate university: Brown University
Research summary
In the Humphreys lab, we use single cell sequencing techniques to study a proximal tubule subpopulation that appears to drive progressive damage and kidney disease. This population has been studied following acute kidney injury (AKI) and is associated with failure to recover and subsequent transition to chronic kidney disease (CKD). I am exploring this subpopulation’s presence and activity in kidneys that have not experienced AKI and whether it can drive CKD progression in other non-acute modes of kidney injury
Graduate publications
Li H, Li D, Ledru N, Xuanyuan Q, Wu H, Asthana A, Byers LN, Tullius SG, Orlando G, Waikar SS, Humphreys BD. 2024 Transcriptomic, epigenomic, and spatial metabolomic cell profiling redefines regional human kidney anatomy. Cell Metab, 36(5):1105-1125.e10.
Ledru N, Wilson PC, Muto Y, Yoshimura Y, Wu H, Li D, Asthana A, Tullius SG, Waikar SS, Orlando G, Humphreys BD. 2024 Predicting proximal tubule failed repair drivers through regularized regression analysis of single cell multiomic sequencing. Nat Commun, 15(1):1291.
Muto Y, Dixon EE, Yoshimura Y, Ledru N, Kirita Y, Wu H, Humphreys BD. 2024 Epigenetic reprogramming driving successful and failed repair in acute kidney injury. Sci Adv, 10(32):39110788.
Yoshimura Y, Muto Y, Ledru N, Wu H, Omachi K, Miner JH, Humphreys BD. 2023 A single-cell multiomic analysis of kidney organoid differentiation. Proc Natl Acad Sci USA, 120(20)::e2219699120.
Muto Y, Dixon EE, Yoshimura Y, Wu H, Omachi K, Ledru N, Wilson PC, King AJ, Eric Olson N, Gunawan MG, Kuo JJ, Cox JH, Miner JH, Seliger SL, Woodward OM, Welling PA, Watnick TJ, Humphreys BD. 2022 Defining cellular complexity in human autosomal dominant polycystic kidney disease by multimodal single cell analysis. Nat Commun, 13(1):6497.
Muto Y, Wilson PC, Ledru N, Wu H, Dimke H, Waikar SS, Humphreys BD. 2021 Single cell transcriptional and chromatin accessibility profiling redefine cellular heterogeneity in the adult human kidney. Nat Commun, 12(1):2190.
Wilson PC, Wu H, Kirita Y, Uchimura K, Ledru N, Rennke HG, Welling PA, Waikar SS, Humphreys BD. 2019 The single-cell transcriptomic landscape of early human diabetic nephropathy. Proc Natl Acad Sci USA, 116(39):19619-19625.