Aleksandra (Sasha) Bourdine
Program: Unspecified
Current advisor:
Undergraduate university: University of Toronto
Research summary
During this rotation, I initiated studies on a potential gain of function variant in the ITK gene identified in a patient with prolonged lymphoproliferation and cytopenias after a presumed viral infection. This included examining exome sequencing data, working with healthy patient control cells to begin development of a flow-based assay to check whether the variant alters ITK function, and flow cytometry. I developed and optimized an intracellular flow cytometry-based panel and assay to detect phosphorylated target proteins of interest. I worked with other members of the laboratory to learn how to use the Aurora spectral flow cytometer and other techniques in the Cooper lab.
Graduate publications