Undergraduate university: Stanford University
Metabolomics, the youngest ‘-omics’ field, has not made the jump to single cell analysis. In Gary Patti’s group this past summer, I worked to build out a pipeline for single cell metabolomics by combining MALDI-mass spectrometry (MS) with high resolution immunofluorescence (IF) imaging. MALDI-MS measures metabolites in a 2D tissue slice, enabling spatial localization of molecules. My project aimed to develop an imaging protocol and software to match this spatial metabolomic data to IF images of the same tissue. This identification would allow one to quantify metabolite levels in specific cell subpopulations and tissue compartments, revealing previously unappreciated spatial patterns of metabolic activity.